Few existing protein-protein interface design methods allow for extensive backbone rearrangements during the design process. There is also a dichotomy between redesign methods, which take advantage of the native interface, and
Here, we propose a new method for designing novel protein reagents that combines advantages of redesign and
Conclusions and Significance
This protocol was implemented within the Rosetta3 software suite. To demonstrate and evaluate this protocol, we have developed a benchmarking set of structures from the PDB with loop-mediated interfaces. This protocol can recover the correct loop-mediated interface in 15 out of 16 tested structures, using only a single residue as an anchor.
Publisher: Public Library of Science
Date Published: 17-June-2011
Author(s): Lewis S., Kuhlman B.