Research Summary: Ascl1 (Mash1) Defines Cells with Long-Term Neurogenic Potential in Subgranular and Subventricular Zones in Adult Mouse Brain


Ascl1 (Mash1) is a bHLH transcription factor essential for neural differentiation
during embryogenesis but its role in adult neurogenesis is less clear. Here we
show that in the adult brain Ascl1 is dynamically expressed during neurogenesis
in the dentate gyrus subgranular zone (SGZ) and more rostral subventricular zone
(SVZ). Specifically, we find Ascl1 levels low in SGZ Type-1 cells and SVZ B
cells but increasing as the cells transition to intermediate progenitor stages.
In vivo genetic lineage tracing with a tamoxifen (TAM) inducible
Ascl1CreERT2 knock-in mouse strain shows
that Ascl1 lineage cells continuously generate new neurons over extended periods
of time. There is a regionally-specific difference in neuron generation, with
mice given TAM at postnatal day 50 showing new dentate gyrus neurons through 30
days post-TAM, but showing new olfactory bulb neurons even 180 days post-TAM.
These results show that Ascl1 is not restricted to transit amplifying
populations but is also found in a subset of neural stem cells with long-term
neurogenic potential in the adult brain.


Publisher: Public Library of Science

Date Published: 31-March-2011

Author(s): Kim E., Ables J., Dickel L., Eisch A., Johnson J.


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