The availability of human neuronal progenitors (hNPs) in high purity would greatly facilitate neuronal drug discovery and developmental studies, as well as cell replacement strategies for neurodegenerative diseases and conditions, such as spinal cord injury, stroke, Parkinson’s disease, Alzheimer’s disease, and Huntington’s disease. Here we describe for the first time a method for producing hNPs in large quantity and high purity from human embryonic stem cells (hESCs) in feeder-free conditions, without the use of exogenous noggin, sonic hedgehog or analogs, rendering the process clinically compliant. The resulting population displays characteristic neuronal-specific markers. When allowed to spontaneously differentiate into neuronal subtypes
Publisher: Public Library of Science
Date Published: 6-June-2011
Author(s): Nistor G., Siegenthaler M., Poirier S., Rossi S., Poole A., Charlton M., McNeish J., Airriess C., Keirstead H.