Beside its effects on T cells, a direct influence on cells of the myelo-monocytic lineage by GA becomes evident. Recently, we demonstrated that GA drives microglia to adopt properties of type II antigen presenting cells (APC) and increases their phagocytic activity. In the present work, we focused on human blood monocytes in order to examine whether GA may increase phagocytic activity
Peripheral blood mononuclear cells (PBMC) were obtained using a Biocoll-Isopaque gradient and monocytes were subsequently isolated by using CD14 MicroBeads. Phagocytic activity was determined by flow cytometric measurement of the ingestion of fluorescent beads. Flow cytometry was also used to assess monocytic differentiation and expression of phagocytic receptors. Monocytes of GA treated MS patients exhibited a significantly higher phagocytic activity than those of healthy controls or non-treated MS patients.
Our results demonstrate a new mechanism of action of GA treatment that augments phagocytic activity of human monocytes
Publisher: Public Library of Science
Date Published: 20-December-2012
Author(s): Pul R., Morbiducci F., Škuljec J., Skripuletz T., Singh V., Diederichs U., Garde N., Voss E., Trebst C., Stangel M.