Whole genome amplification and sequencing of single microbial cells has significantly influenced genomics and microbial ecology by facilitating direct recovery of reference genome data. However, viral genomics continues to suffer due to difficulties related to the isolation and characterization of uncultivated viruses. We report here on a new approach called ‘Single Virus Genomics’, which enabled the isolation and complete genome sequencing of the first single virus particle. A mixed assemblage comprised of two known viruses;
Publisher: Public Library of Science
Date Published: 23-March-2011
Author(s): Allen L., Ishoey T., Novotny M., McLean J., Lasken R., Williamson S.