Galectin-3 (Gal-3) is an anti-apoptotic molecule involved in thyroid cells transformation. It is specifically overexpressed in thyroid tumour cells and is currently used as a preoperative diagnostic marker of thyroid malignancy. Gal-3 expression is downregulated by wt-p53 at the transcriptional level. In well-differentiated thyroid carcinomas (WDTCs) there is an unexplained paradoxical concomitant expression of Gal-3 and wt-p53. HIPK2 is a co-regulator of different transcription factors, and modulates basic cellular processes mainly through the activation of wt-p53. Since we demonstrated that HIPK2 is involved in p53-mediated Gal-3 downregulation, we asked whether HIPK2 deficiency might be responsible for such paradoxical Gal-3 overexpression in WDTC.
We analyzed HIPK2 protein and mRNA levels, as well as loss of heterozygosity (LOH) at the
Our results indicate that HIPK2 expression and function are impaired in WDTCs, in particular in PTCs, and that this event explains Gal-3 overexpression typically observed in these types of tumours. Therefore,
Publisher: Public Library of Science
Date Published: 17-June-2011
Author(s): Lavra L., Rinaldo C., Ulivieri A., Luciani E., Fidanza P., Giacomelli L., Bellotti C., Ricci A., Trovato M., Soddu S., Bartolazzi A., Sciacchitano S.